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Text File | 1995-03-07 | 21.7 KB | 754 lines | [TEXT/KrOt] |
- #Three-dimensional structure of the HIV-1 matrix protein p17.
- #
- #This molecular structure was recently determined by nuclear
- #magnetic resonance (NMR) at Oxford University.
- #
- #Contributed by doak@bioch.ox.ac.uk
- #
- #Further details and reference in file comments.
- #
- #dots added by craig@raru.adelaide.edu.au 24 Sep 94
-
- #The human immunodeficiency virus (HIV) matrix protein, p17, forms the outer
- #shell of the core of the virus, lining the inner surface of the viral membrane. The
- #protein has several key functions. It orchestrates viral assembly via targeting
- #signals that direct the gag precursor polyprotein, p55, to the host cell membrane
- #and it interacts with the transmembrane protein, gp41, to retain the env
- #-encoded proteins in the virus. In addition, p17 contains a nuclear localisation
- #signal that directs the pre-integration complex to the nucleus of infected cells.
- #This permits the virus to infect productively non-dividing cells, a distinguishing
- #feature of HIV and other lentiviruses. We have determined the solution
- #structure of p17 by nuclear magnetic resonance (NMR) with a root-mean
- #square deviation for the backbone of the well defined regions of 0.9≈. It consists
- #of four helices connected by short loops and an irregular, mixed beta sheet that
- #provides a positively charged surface for interaction with the inner layer of the
- #membrane. The helical topology is unusual; the Brookhaven protein database
- #contains only one similar structure, that of the immune modulator
- #interferon-gamma.
- #
- #Stephen Matthews, Paul Barlow, Jonathan Boyd, Geoff Barton, Robert
- #Russell, Helen Mills, Mark Cunningham, Nicola Meyers, Nigel Burns, Nigel
- #Clark, Susan Kingsman, Alan Kingsman and Iain Campbell.
- #
- #Reference: Nature, 370:666-668, 1994.
- #
- #See also: http://nmra.ocms.ox.ac.uk/
- #
- #The xyz coordinates in this file were generated from a pdb file using a simple perl
- #script. I have found Craig Kloeden's Rotater program to be an excellent,
- #cheap (free!) method of displaying protein structures on my Mac.
- #
- # D.Doak
- #
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